OligoExome Statistics

Parameter Human OligoExome
Total number of genes from CCDS 17,049
Total number of ROI sequences 157,624
Average ROIs per gene 9.25
Total ROI bases derived from CCDS (Mb) 44.6
Total number of designed capture oligonucleotides 784,783
Number of captured ROI bases by design for all restriction enzymes 98.30%
Average number of capture oligonucleotides per gene 46
Average number of capture oligonucleotides per ROI 5
Region-of-interest(ROI) is defined as a minimum of the exon and adjacent intronic sequence up to 50 bases from the exon flank

Restriction enzyme BfaI CviQI MseI Sau3AI Total per category
Total designed capture oligonucleotides 190,900 191,315 186,011 216,557 784,783
Oligonucleotides with no flag 169,776 171,320 161,813 184,754 687,663
Oligonucleotides - whole genome (W-flag) 20,992 19,882 24,021 31,670 96,565
Oligonucleotides - paralog (P-flag) 4,915 4,983 4,854 6,179 20,931
Oligonucleotides - Alu (A-flag) 290 183 58 460 991
Oligonucleotides - repeat sequence (R-flag) 379 379 578 456 1,792
Total flagged capture oligonucleotides per restriction enzyme (union of all flagged oligos) 21,124 19,995 24,198 31,803 97,120

Capture oligonucleotide enzymes BfaI, CviQI, MseI, Sau3AI BfaI, CviQI, MseI
Number of enzymes 4 3
Total ROI target of CCDS (Mb) 44.6 44.6
Filtering with all flags No Yes No Yes
Percentage of total ROI target covered by 1 capture oligonucleotide 98.30% 94.20% 95.90% 91.10%
Percentage of total ROI target with greater than 1 capture oligonucleotide 91.90% 83.90% 81.70% 72.20%
Percentage of ROI target missed by exome design 1.70% 5.80% 4.10% 8.90%
Filtering with all flags includes the W-flag (whole genome matches), P-flag (paralogs), A-flag (Alu) and R-flag (other repeats)

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